CYLD
Gene Ontology Biological Process
- necroptotic process [IGI]
- negative regulation of NF-kappaB import into nucleus [ISO]
- negative regulation of NF-kappaB transcription factor activity [IBA, ISO]
- negative regulation of T cell differentiation [IDA]
- negative regulation of canonical Wnt signaling pathway [ISO]
- positive regulation of extrinsic apoptotic signaling pathway [IBA, ISO]
- protein K63-linked deubiquitination [IBA, ISO]
- protein deubiquitination [IMP]
- proteolysis [IBA]
- regulation of intrinsic apoptotic signaling pathway [IBA, ISO]
- regulation of microtubule cytoskeleton organization [ISO]
- regulation of mitotic cell cycle [IBA, ISO]
- ripoptosome assembly involved in necroptotic process [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ITCH
Gene Ontology Biological Process
- negative regulation of JNK cascade [IMP]
- negative regulation of NF-kappaB transcription factor activity [IMP]
- negative regulation of alpha-beta T cell proliferation [IMP]
- negative regulation of apoptotic process [ISO]
- negative regulation of defense response to virus [IMP, ISO]
- positive regulation of T cell anergy [IMP]
- positive regulation of protein catabolic process [IDA, IMP]
- protein K29-linked ubiquitination [ISO]
- protein K48-linked ubiquitination [IMP, ISO]
- protein K63-linked ubiquitination [ISO]
- protein polyubiquitination [IDA]
- protein ubiquitination [IBA, ISO]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA]
- regulation of protein deubiquitination [IMP]
- ubiquitin-dependent protein catabolic process [IDA, ISA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Ubiquitin E3 ligase Itch negatively regulates osteoclast formation by promoting de-ubiquitination of TNF receptor-associated factor 6 (TRAF6).
Itch is an ubiquitin E3 ligase that regulates protein stability. Itch-/- mice develop an autoimmune disease phenotype characterized by Itchy skin and multi-organ inflammation. However, the role of Itch in the regulation of osteoclast function has not been examined. We report that Itch-/- bone marrow and spleen cells formed more osteoclasts than cells from WT littermates in response to RANKL, ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ITCH CYLD | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CYLD ITCH | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID