PAK1
Gene Ontology Biological Process
- MAPK cascade [ISO, TAS]
- actin cytoskeleton reorganization [ISO, ISS]
- activation of MAPKK activity [TAS]
- axonogenesis [IBA]
- branching morphogenesis of an epithelial tube [ISO, ISS]
- cellular response to insulin stimulus [IDA]
- dendrite development [ISO]
- intracellular signal transduction [TAS]
- mitotic cell cycle [IBA]
- negative regulation of cell proliferation involved in contact inhibition [ISO, ISS]
- neuromuscular junction development [ISO]
- neuron projection morphogenesis [IMP]
- positive regulation of JUN kinase activity [ISO, ISS]
- positive regulation of intracellular estrogen receptor signaling pathway [ISO, ISS]
- positive regulation of peptidyl-serine phosphorylation [ISO, ISS]
- positive regulation of protein phosphorylation [ISO, ISS]
- positive regulation of stress fiber assembly [ISO, ISS]
- protein autophosphorylation [IDA, ISO, ISS]
- protein phosphorylation [IDA, ISO, ISS, TAS]
- receptor clustering [ISO]
- regulation of actin cytoskeleton organization [IMP]
- response to hypoxia [IDA]
- response to organic substance [IDA]
- small GTPase mediated signal transduction [TAS]
- wound healing [ISO, ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [ISO, ISS]
- Z disc [IDA]
- axon [IDA, ISO]
- cell-cell junction [ISO, ISS]
- cytoplasm [ISO, ISS]
- cytosol [IDA]
- dendrite [IDA, ISO]
- growth cone [ISO]
- intercalated disc [IDA]
- membrane [IDA]
- neuronal postsynaptic density [ISO]
- nuclear membrane [IDA]
- nucleus [ISO]
- plasma membrane [IDA, ISO, ISS]
- protein complex [ISO]
- ruffle [ISO, ISS]
CDC42
Gene Ontology Biological Process
- GTP catabolic process [TAS]
- Golgi organization [IMP]
- actin cytoskeleton organization [ISO]
- actin filament branching [IMP]
- actin filament bundle assembly [ISO]
- actin filament organization [IMP]
- adherens junction organization [ISO]
- canonical Wnt signaling pathway [ISO]
- cardiac conduction system development [ISO]
- cellular protein localization [ISO]
- dendritic cell migration [ISO]
- epidermis morphogenesis [ISO]
- epithelial cell-cell adhesion [ISO]
- epithelial-mesenchymal cell signaling [ISO]
- establishment of Golgi localization [IMP]
- establishment of cell polarity [TAS]
- establishment or maintenance of apical/basal cell polarity [ISO]
- filopodium assembly [ISO]
- hair follicle morphogenesis [ISO]
- hair follicle placode formation [ISO]
- heart contraction [ISO]
- keratinization [ISO]
- keratinocyte development [ISO]
- multicellular organism growth [ISO]
- negative regulation of gene expression [ISO]
- negative regulation of protein complex assembly [ISO]
- neuron fate determination [ISO]
- nuclear migration [ISO]
- nucleus localization [ISO]
- organelle transport along microtubule [IMP]
- positive regulation of DNA replication [IMP]
- positive regulation of JNK cascade [IDA]
- positive regulation of MAPK cascade [ISO]
- positive regulation of cytokinesis [ISO, ISS]
- positive regulation of gene expression [ISO]
- positive regulation of hair follicle cell proliferation [ISO]
- positive regulation of intracellular protein transport [IMP]
- positive regulation of metalloenzyme activity [IMP]
- positive regulation of neuron apoptotic process [IMP]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of phosphatidylinositol 3-kinase activity [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of pseudopodium assembly [ISO]
- positive regulation of substrate adhesion-dependent cell spreading [ISO, ISS]
- positive regulation of synapse structural plasticity [IMP]
- regulation of attachment of spindle microtubules to kinetochore [ISO, ISS]
- regulation of exocytosis [TAS]
- regulation of filopodium assembly [ISO, ISS]
- regulation of mitosis [ISO]
- regulation of protein catabolic process [ISO]
- regulation of protein heterodimerization activity [ISO]
- regulation of protein kinase activity [ISO]
- regulation of protein metabolic process [ISO]
- regulation of protein stability [ISO]
- response to glucose [TAS]
- single organismal cell-cell adhesion [ISO]
- sprouting angiogenesis [IMP]
- submandibular salivary gland formation [IEP]
- substantia nigra development [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi membrane [IDA]
- apical part of cell [ISO]
- cell [ISO]
- cell periphery [ISO]
- cell projection [ISO]
- cell-cell junction [ISO]
- cytoplasm [ISO]
- cytoplasmic ribonucleoprotein granule [ISO]
- cytosol [TAS]
- extracellular vesicular exosome [ISO]
- filopodium [ISO]
- focal adhesion [ISO]
- leading edge membrane [ISO]
- membrane [ISO, ISS]
- midbody [ISO, ISS]
- mitotic spindle [ISO, ISS]
- myelin sheath [ISO]
- neuron projection [ISO]
- neuronal cell body [ISO]
- plasma membrane [IDA, ISO]
- secretory granule [IDA]
- spindle midzone [ISO, ISS]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Identification and molecular cloning of a p21cdc42/rac1-activated serine/threonine kinase that is rapidly activated by thrombin in platelets.
The brain-enriched p21cdc42/rac1-activated serine/threonine kinase, p65PAK, was identified and purified on the basis of overlays with [gamma-32P]GTP-Cdc42 onto SDS-fractionated proteins (Manser, E., Leung, T., Salihuddin, H., Zhao, Z.-S., and Lim, L. (1994) Nature 367, 40-46). In this study, the ubiquitously expressed p21cdc42/rac1 binding protein with relative molecular weight of 62,000 was purified from rat testes and shown to contain peptides ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PAK1 CDC42 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID