SQSTM1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
IRS1
Gene Ontology Biological Process
- cellular response to insulin stimulus [ISO]
- insulin receptor signaling pathway [IDA, ISO]
- insulin secretion [ISO]
- insulin-like growth factor receptor signaling pathway [ISO, ISS]
- lipid catabolic process [ISO]
- mammary gland development [ISO]
- negative regulation of insulin receptor signaling pathway [IMP]
- negative regulation of insulin secretion [IMP, ISO]
- negative regulation of somatostatin secretion [IMP]
- phosphatidylinositol 3-kinase signaling [ISO]
- positive regulation of cell migration [ISO]
- positive regulation of fatty acid beta-oxidation [ISO]
- positive regulation of glucagon secretion [IMP]
- positive regulation of glucose import [ISO]
- positive regulation of glucose import in response to insulin stimulus [ISO]
- positive regulation of glucose metabolic process [ISO]
- positive regulation of glycogen biosynthetic process [ISO]
- positive regulation of insulin receptor signaling pathway [IMP]
- positive regulation of mesenchymal cell proliferation [ISO]
- positive regulation of phosphatidylinositol 3-kinase activity [IDA, ISO]
- positive regulation of phosphorylation [IDA]
- protein heterooligomerization [IDA]
- protein kinase B signaling [ISO]
- protein localization to nucleus [ISO]
- regulation of gene expression [ISO]
- response to caffeine [IDA]
- response to insulin [ISO]
- response to peptide hormone [IDA]
Gene Ontology Molecular Function- SH2 domain binding [IPI]
- insulin receptor binding [IMP, ISO, ISS]
- insulin-like growth factor receptor binding [IPI, ISO, ISS]
- phosphatidylinositol 3-kinase binding [IDA, IMP, IPI, ISO]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein kinase C binding [IPI]
- protein kinase binding [IPI, ISO]
- signal transducer activity [IBA]
- transmembrane receptor protein tyrosine kinase adaptor activity [IMP]
- SH2 domain binding [IPI]
- insulin receptor binding [IMP, ISO, ISS]
- insulin-like growth factor receptor binding [IPI, ISO, ISS]
- phosphatidylinositol 3-kinase binding [IDA, IMP, IPI, ISO]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein kinase C binding [IPI]
- protein kinase binding [IPI, ISO]
- signal transducer activity [IBA]
- transmembrane receptor protein tyrosine kinase adaptor activity [IMP]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Sequestosome 1/p62, a scaffolding protein, is a newly identified partner of IRS-1 protein.
Defects in the insulin-signaling pathway may lead to the development of skeletal muscle insulin resistance, which is one of the earliest abnormalities detected in individuals with the metabolic syndrome and predisposes them to develop type 2 diabetes. Previous studies have shown that deletion of the mouse sequestosome 1/p62 gene results in mature-onset obesity that progresses to insulin and leptin resistance ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| IRS1 SQSTM1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SQSTM1 IRS1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID