BAIT
ADRB2
ADRB2R, ADRBR, B2AR, BAR, BETA2AR
adrenoceptor beta 2, surface
GO Process (10)
GO Function (5)
GO Component (5)
Gene Ontology Biological Process
- activation of adenylate cyclase activity [IDA]
- activation of transmembrane receptor protein tyrosine kinase activity [TAS]
- adenylate cyclase-modulating G-protein coupled receptor signaling pathway [TAS]
- adrenergic receptor signaling pathway [IDA]
- cell surface receptor signaling pathway [TAS]
- desensitization of G-protein coupled receptor protein signaling pathway by arrestin [IDA]
- endosome to lysosome transport [TAS]
- positive regulation of MAPK cascade [IDA]
- positive regulation of protein ubiquitination [IMP]
- receptor-mediated endocytosis [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ATP1A1
RP4-655J12.1
ATPase, Na+/K+ transporting, alpha 1 polypeptide
GO Process (14)
GO Function (4)
GO Component (10)
Gene Ontology Biological Process
- cardiac muscle contraction [TAS]
- cell communication by electrical coupling involved in cardiac conduction [TAS]
- cellular potassium ion homeostasis [IDA]
- cellular response to steroid hormone stimulus [IDA]
- cellular sodium ion homeostasis [IDA]
- ion transmembrane transport [TAS]
- membrane repolarization [IDA]
- membrane repolarization during cardiac muscle cell action potential [IC]
- potassium ion import [IDA]
- regulation of sodium ion transport [ISS]
- relaxation of cardiac muscle [TAS]
- response to glycoside [IDA]
- sodium ion export from cell [IDA]
- transmembrane transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Novel, gel-free proteomics approach identifies RNF5 and JAMP as modulators of GPCR stability.
The maturation and folding of G protein-coupled receptors (GPCRs) are governed by mechanisms that remain poorly understood. In an effort to characterize these biological events, we optimized a novel, gel-free proteomic approach to identify partners of the β2-adrenergic receptor (β2AR). In addition to a number of known interacting proteins such as heterotrimeric G protein subunits, this allowed us to identify ... [more]
Mol. Endocrinol. Jun. 24, 2013; 0(0); [Pubmed: 23798571]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293 cell (BTO:0000007)
Additional Notes
- exogenous expression of bait
Curated By
- BioGRID