PREY

LMNB1

ADLD, LMN, LMN2, LMNB
lamin B1
GO Process (2)
GO Function (0)
GO Component (4)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Novel, gel-free proteomics approach identifies RNF5 and JAMP as modulators of GPCR stability.

Roy SJ, Glazkova I, Frechette L, Iorio-Morin C, Binda C, Petrin D, Trieu P, Robitaille M, Angers S, Hebert TE, Parent JL

The maturation and folding of G protein-coupled receptors (GPCRs) are governed by mechanisms that remain poorly understood. In an effort to characterize these biological events, we optimized a novel, gel-free proteomic approach to identify partners of the β2-adrenergic receptor (β2AR). In addition to a number of known interacting proteins such as heterotrimeric G protein subunits, this allowed us to identify ... [more]

Mol. Endocrinol. Jun. 24, 2013; 0(0); [Pubmed: 23798571]

Throughput

  • High Throughput

Ontology Terms

  • cell line: hek-293 cell (BTO:0000007)

Additional Notes

  • exogenous expression of bait

Curated By

  • BioGRID