BAIT
ADRB2
ADRB2R, ADRBR, B2AR, BAR, BETA2AR
adrenoceptor beta 2, surface
GO Process (10)
GO Function (5)
GO Component (5)
Gene Ontology Biological Process
- activation of adenylate cyclase activity [IDA]
- activation of transmembrane receptor protein tyrosine kinase activity [TAS]
- adenylate cyclase-modulating G-protein coupled receptor signaling pathway [TAS]
- adrenergic receptor signaling pathway [IDA]
- cell surface receptor signaling pathway [TAS]
- desensitization of G-protein coupled receptor protein signaling pathway by arrestin [IDA]
- endosome to lysosome transport [TAS]
- positive regulation of MAPK cascade [IDA]
- positive regulation of protein ubiquitination [IMP]
- receptor-mediated endocytosis [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
TTN
CMD1G, CMH9, CMPD4, EOMFC, HMERF, LGMD2J, MYLK5, TMD
titin
GO Process (20)
GO Function (15)
GO Component (8)
Gene Ontology Biological Process
- blood coagulation [TAS]
- cardiac muscle contraction [IMP]
- cardiac muscle fiber development [IMP]
- cardiac muscle hypertrophy [IMP]
- cardiac muscle tissue morphogenesis [IMP]
- cardiac myofibril assembly [IMP]
- detection of muscle stretch [TAS]
- mitotic chromosome condensation [IEP]
- muscle contraction [NAS, TAS]
- muscle filament sliding [TAS]
- platelet activation [TAS]
- platelet degranulation [TAS]
- regulation of catalytic activity [IMP]
- regulation of protein kinase activity [IMP]
- response to calcium ion [IDA]
- sarcomere organization [IMP]
- sarcomerogenesis [IMP]
- skeletal muscle myosin thick filament assembly [IMP]
- skeletal muscle thin filament assembly [IMP]
- striated muscle contraction [TAS]
Gene Ontology Molecular Function- actin filament binding [IDA]
- actinin binding [IDA, IPI]
- calcium ion binding [IDA]
- calmodulin binding [IPI, TAS]
- enzyme binding [IPI]
- identical protein binding [IPI]
- muscle alpha-actinin binding [IPI]
- protease binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein self-association [IDA]
- protein serine/threonine kinase activity [IDA]
- structural constituent of muscle [IMP, TAS]
- structural molecule activity conferring elasticity [TAS]
- telethonin binding [IPI, ISS]
- actin filament binding [IDA]
- actinin binding [IDA, IPI]
- calcium ion binding [IDA]
- calmodulin binding [IPI, TAS]
- enzyme binding [IPI]
- identical protein binding [IPI]
- muscle alpha-actinin binding [IPI]
- protease binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein self-association [IDA]
- protein serine/threonine kinase activity [IDA]
- structural constituent of muscle [IMP, TAS]
- structural molecule activity conferring elasticity [TAS]
- telethonin binding [IPI, ISS]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Novel, gel-free proteomics approach identifies RNF5 and JAMP as modulators of GPCR stability.
The maturation and folding of G protein-coupled receptors (GPCRs) are governed by mechanisms that remain poorly understood. In an effort to characterize these biological events, we optimized a novel, gel-free proteomic approach to identify partners of the β2-adrenergic receptor (β2AR). In addition to a number of known interacting proteins such as heterotrimeric G protein subunits, this allowed us to identify ... [more]
Mol. Endocrinol. Jun. 24, 2013; 0(0); [Pubmed: 23798571]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293 cell (BTO:0000007)
Additional Notes
- exogenous expression of bait
Curated By
- BioGRID