An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.

Publication

BAG3 Interactome Analysis Reveals a New Role in Modulating Proteasome Activity.

Chen Y, Yang LN, Cheng L, Tu S, Guo SJ, Le HY, Xiong Q, Mo R, Li CY, Jeong JS, Jiang L, Blackshaw S, Bi LJ, Zhu H, Tao SC, Ge F

Bcl2-associated athanogene 3 (BAG3), a member of the BAG family of co-chaperones, plays a critical role in regulating apoptosis, development, cell motility, autophagy, and tumor metastasis, and in mediating cell adaptive responses to stressful stimuli. BAG3 carries a BAG domain, a WW domain and a proline-rich (PXXP) repeat, all of which mediate binding to different partners. To elucidate BAG3 interaction ... [more]

Mol. Cell Proteomics Jul. 03, 2013; 0(0); [Pubmed: 23824909]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
KRT19 BAG3	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Go CD (2021)	High	18.26	BioGRID	2991939

Curated By

BioGRID

Interaction Summary

BAG3

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

KRT19