An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Binding of protein kinase B to the plakin family member periplakin.

van den Heuvel AP, de Vries-Smits AM, van Weeren PC, Dijkers PF, de Bruyn KM, Riedl JA, Burgering BM

The serine/threonine kinase protein kinase B (PKB/c-Akt) acts downstream of the lipid kinase phosphoinositide 3-kinase (PI3K) and functions as an essential mediator in many growth-factor-induced cellular responses such as cell cycle regulation, cell survival and transcriptional regulation. PI3K activation generates 3'-phosphorylated phosphatidylinositol lipids (PtdIns3P) and PKB activation requires PtdIns3P-dependent membrane translocation and phosphorylation by upstream kinases. However PKB activation and ... [more]

J. Cell. Sci. Oct. 15, 2002; 115(0);3957-66 [Pubmed: 12244133]

Throughput

Low Throughput

Ontology Terms

mcf-7 cell (BTO:0000093)
female breast cancer (DOID:0050671)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
VIM PPL	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	van den Heuvel AP (2002)	Low	-	BioGRID	878814
PPL VIM	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Kazerounian S (2002)	Low	-	BioGRID	-
PPL VIM	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Rolland T (2014)	High	-	BioGRID	-
PPL VIM	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	van den Heuvel AP (2002)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

PPL

Gene Ontology Molecular Function

protein binding [IPI]structural constituent of cytoskeleton [TAS]

Gene Ontology Cellular Component

VIM

Gene Ontology Biological Process

Gene Ontology Molecular Function

double-stranded RNA binding [IDA]glycoprotein binding [IPI]identical protein binding [IPI]protein C-terminus binding [IPI]protein binding [IPI]scaffold protein binding [IPI]structural constituent of cytoskeleton [IDA]