Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

DTF1 is a core component of RNA-directed DNA methylation and may assist in the recruitment of Pol IV.

Zhang H, Ma ZY, Zeng L, Tanaka K, Zhang CJ, Ma J, Bai G, Wang P, Zhang SW, Liu ZW, Cai T, Tang K, Liu R, Shi X, He XJ, Zhu JK

DNA methylation is an important epigenetic mark in many eukaryotic organisms. De novo DNA methylation in plants can be achieved by the RNA-directed DNA methylation (RdDM) pathway, where the plant-specific DNA-dependent RNA polymerase IV (Pol IV) transcribes target sequences to initiate 24-nt siRNA production and action. The putative DNA binding protein DTF1/SHH1 of Arabidopsis has been shown to associate with ... [more]

Proc. Natl. Acad. Sci. U.S.A. May. 14, 2013; 110(20);8290-5 [Pubmed: 23637343]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AT1G15215 RDR2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Zhang H (2013)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RDR2

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA-directed RNA polymerase activity [ISS]

Gene Ontology Cellular Component

AT1G15215