PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

The Identification of Novel Proteins That Interact With the GLP-1 Receptor and Restrain its Activity.

Huang X, Dai FF, Gaisano G, Giglou K, Han J, Zhang M, Kittanakom S, Wong V, Wei L, Showalter AD, Sloop KW, Stagljar I, Wheeler MB

Glucagon-like peptide 1 receptor (GLP-1R) controls diverse physiological functions in tissues, including pancreatic islets, the brain, and the heart. To understand the mechanisms that control glucagon-like peptide 1 (GLP-1) signaling better, we sought to identify proteins that interact with the GLP-1R using a membrane-based split ubiquitin yeast two-hybrid (MYTH) assay. A screen of a human fetal brain cDNA prey library ... [more]

Mol. Endocrinol. Jul. 17, 2013; 0(0); [Pubmed: 23864651]

Throughput

  • Low Throughput

Curated By

  • BioGRID