GLP1R
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
HPN
Gene Ontology Biological Process
- basement membrane disassembly [IDA]
- cochlea morphogenesis [ISS]
- detection of mechanical stimulus involved in sensory perception of sound [ISS]
- negative regulation of apoptotic process [IDA]
- negative regulation of epithelial cell proliferation [IDA]
- negative regulation of epithelial to mesenchymal transition [IDA]
- pilomotor reflex [ISS]
- positive regulation by host of viral transcription [IDA]
- positive regulation of cell growth [IMP]
- positive regulation of gene expression [ISS]
- positive regulation of hepatocyte proliferation [IDA]
- positive regulation of plasminogen activation [IDA]
- positive regulation of thyroid hormone generation [ISS]
- potassium ion transmembrane transport [ISS]
- proteolysis [IDA]
- regulation of cell shape [IMP]
- response to thyroid hormone [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The Identification of Novel Proteins That Interact With the GLP-1 Receptor and Restrain its Activity.
Glucagon-like peptide 1 receptor (GLP-1R) controls diverse physiological functions in tissues, including pancreatic islets, the brain, and the heart. To understand the mechanisms that control glucagon-like peptide 1 (GLP-1) signaling better, we sought to identify proteins that interact with the GLP-1R using a membrane-based split ubiquitin yeast two-hybrid (MYTH) assay. A screen of a human fetal brain cDNA prey library ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GLP1R HPN | PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay. | Low | - | BioGRID | - |
Curated By
- BioGRID