BAIT

PELI3

pellino E3 ubiquitin protein ligase family member 3
GO Process (2)
GO Function (0)
GO Component (1)
Homo sapiens
PREY

UBE2V1

CIR1, CROC-1, CROC1, UBE2V, UEV-1, UEV1, UEV1A, P/OKcl.19
ubiquitin-conjugating enzyme E2 variant 1
GO Process (23)
GO Function (4)
GO Component (8)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Pellino3 ubiquitinates RIP2 and mediates Nod2-induced signaling and protective effects in colitis.

Yang S, Wang B, Humphries F, Jackson R, Healy ME, Bergin R, Aviello G, Hall B, McNamara D, Darby T, Quinlan A, Shanahan F, Melgar S, Fallon PG, Moynagh PN

Mutations that result in loss of function of Nod2, an intracellular receptor for bacterial peptidoglycan, are associated with Crohn's disease. Here we found that the E3 ubiquitin ligase Pellino3 was an important mediator in the Nod2 signaling pathway. Pellino3-deficient mice had less induction of cytokines after engagement of Nod2 and had exacerbated disease in various experimental models of colitis. Furthermore, ... [more]

Nat. Immunol. Jul. 28, 2013; 0(0); [Pubmed: 23892723]

Throughput

  • Low Throughput

Ontology Terms

  • hek-293t cell (BTO:0002181)

Additional Notes

  • E1-
  • E2- UbcH13-Uev1a
  • Pellino3 catalyzes K63-linked polyubiquitination of RIP2

Curated By

  • BioGRID