Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

DEAF1 is a Pellino1-interacting protein required for interferon production by Sendai virus and double stranded RNA.

Ordureau A, Enesa K, Nanda S, Le Francois B, Peggie M, Prescott A, Albert PR, Cohen P

Double-stranded (ds) RNA of viral origin, a ligand for Melanoma Differentiation Associated gene 5 (MDA5) and Toll-Like Receptor 3 (TLR3), induces the TANK-Binding Kinase 1 (TBK1) -dependent phosphorylation and activation of Interferon Regulatory Factor 3 (IRF3) and the E3 ubiquitin ligase Pellino1, which are required for interferon β (IFNβ) gene transcription. Here, we report that Pellino1 interacts with the transcription ... [more]

J. Biol. Chem. Jul. 11, 2013; 0(0); [Pubmed: 23846693]

Throughput

  • Low Throughput

Ontology Terms

  • hek-293ft cell (BTO:0005029)

Curated By

  • BioGRID