BAIT

STIP1

Hop, Sti1, p60
stress-induced phosphoprotein 1
GO Process (0)
GO Function (5)
GO Component (2)
Mus musculus
PREY

MLLT3

2210011H10Rik, 2610012I03Rik, 3830408D16Rik, Af9, D4Ertd321e, RP23-440D4.1
myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Hsp90 directly modulates the spatial distribution of AF9/MLLT3 and affects target gene expression.

Lin JJ, Hemenway CS

AF9/MLLT3 contributes to the regulation of the gene encoding the epithelial sodium channel alpha, ENaCalpha, in renal tubular cells. Specifically, increases in AF9 protein lead to a reduction in ENaCalpha expression and changes in AF9 activity appear to be an important component of aldosterone signaling in the kidney. Whereas AF9 is found in the nucleus where it interacts with the ... [more]

J. Biol. Chem. Apr. 16, 2010; 285(16);11966-73 [Pubmed: 20159978]

Throughput

  • Low Throughput

Curated By

  • BioGRID