CRKL
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
INPP5D
Gene Ontology Biological Process
- dephosphorylation [IDA]
- determination of adult lifespan [IMP]
- immunoglobulin mediated immune response [IMP]
- intracellular signal transduction [IMP]
- negative regulation of B cell activation [IMP]
- negative regulation of B cell proliferation [IMP]
- negative regulation of bone resorption [IMP]
- negative regulation of cell proliferation [IDA]
- negative regulation of granulocyte differentiation [IMP]
- negative regulation of immune response [IMP]
- negative regulation of interleukin-6 biosynthetic process [IMP]
- negative regulation of monocyte differentiation [IMP]
- negative regulation of neutrophil differentiation [IMP]
- negative regulation of osteoclast differentiation [IMP]
- negative regulation of signal transduction [IMP]
- positive regulation of B cell differentiation [IMP]
- positive regulation of apoptotic process [IMP]
- positive regulation of erythrocyte differentiation [IMP]
- positive regulation of lymphocyte differentiation [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
SHIP1, an SH2 domain containing polyinositol-5-phosphatase, regulates migration through two critical tyrosine residues and forms a novel signaling complex with DOK1 and CRKL.
SHIP1 is an SH2 domain containing inositol-5-phosphatase that appears to be a negative regulator of hematopoiesis. The tyrosine kinase oncogene BCR/ABL drastically reduces expression of SHIP1. The major effect of re-expressing SHIP1 in BCR/ABL-transformed cells is reduction of hypermotility. To investigate the potential signaling pathways involving SHIP1 in hematopoietic cells, we overexpressed SHIP1 in a murine BCR/ABL-transformed Ba/F3 cell line ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| INPP5D CRKL | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CRKL INPP5D | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| INPP5D CRKL | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID