BAIT

ARE2

SAT1, L000003220, L000002769, YNR019W
Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

SSM4

DOA10, KIS3, E3 ubiquitin-protein ligase SSM4, L000002087, YIL030C
Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
UPS Project
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Sterol homeostasis requires regulated degradation of squalene monooxygenase by the ubiquitin ligase Doa10/Teb4.

Foresti O, Ruggiano A, Hannibal-Bach HK, Ejsing CS, Carvalho P

Sterol homeostasis is essential for the function of cellular membranes and requires feedback inhibition of HMGR, a rate-limiting enzyme of the mevalonate pathway. As HMGR acts at the beginning of the pathway, its regulation affects the synthesis of sterols and of other essential mevalonate-derived metabolites, such as ubiquinone or dolichol. Here, we describe a novel, evolutionarily conserved feedback system operating ... [more]

Elife Jul. 31, 2013; 2(0);e00953 [Pubmed: 23898401]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)
  • phenotype: cold sensitivity (APO:0000148)
  • phenotype: resistance to chemicals (APO:0000087)

Additional Notes

  • are1 are2 ssm4 triple null mutant is inviable at 14 deg C, or at 25 deg C in the presence of benzyl alcohol

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ARE2 SSM4
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
883100

Curated By

  • BioGRID