BAIT

ARE1

SAT2, L000003221, L000002768, YCR048W
Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication
GO Process (1)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

UBC7

DER2, QRI8, E2 ubiquitin-conjugating protein UBC7, L000001552, YMR022W
Ubiquitin conjugating enzyme; involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
UPS Project
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Sterol homeostasis requires regulated degradation of squalene monooxygenase by the ubiquitin ligase Doa10/Teb4.

Foresti O, Ruggiano A, Hannibal-Bach HK, Ejsing CS, Carvalho P

Sterol homeostasis is essential for the function of cellular membranes and requires feedback inhibition of HMGR, a rate-limiting enzyme of the mevalonate pathway. As HMGR acts at the beginning of the pathway, its regulation affects the synthesis of sterols and of other essential mevalonate-derived metabolites, such as ubiquinone or dolichol. Here, we describe a novel, evolutionarily conserved feedback system operating ... [more]

Elife Jul. 31, 2013; 2(0);e00953 [Pubmed: 23898401]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)
  • phenotype: cold sensitivity (APO:0000148)
  • phenotype: resistance to chemicals (APO:0000087)

Additional Notes

  • are1 are2 ubc7 triple null mutant is inviable at 14 deg C, or at 25 deg C in the presence of benzyl alcohol; data not shown

Curated By

  • BioGRID