BAIT
NANOG
2410002E02Rik, ENK, ecat4
Nanog homeobox
GO Process (22)
GO Function (8)
GO Component (3)
Gene Ontology Biological Process
- cell proliferation [ISO]
- embryo development [IMP]
- endodermal cell fate specification [ISO]
- mesodermal cell fate commitment [IGI]
- negative regulation of BMP signaling pathway [IDA]
- negative regulation of cell differentiation [IDA]
- negative regulation of cell fate commitment [IDA, IGI]
- negative regulation of endodermal cell fate specification [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IGI, IMP, ISO]
- positive regulation of cell proliferation [IDA]
- positive regulation of mitotic cell cycle [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IMP, ISO]
- positive regulation of transcription, DNA-templated [IMP]
- regulation of cell differentiation [ISO]
- regulation of gene expression [ISO]
- regulation of transcription, DNA-templated [ISO]
- response to organic substance [IDA]
- response to retinoic acid [IDA]
- somatic stem cell maintenance [ISO]
- stem cell differentiation [IDA, IMP]
- stem cell division [IDA]
- stem cell maintenance [IDA, IMP]
Gene Ontology Molecular Function- DNA binding [IDA, ISO]
- chromatin binding [IDA]
- enhancer sequence-specific DNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IMP, ISO]
- transcription corepressor activity [IMP]
- transcription regulatory region DNA binding [IDA]
- DNA binding [IDA, ISO]
- chromatin binding [IDA]
- enhancer sequence-specific DNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IMP, ISO]
- transcription corepressor activity [IMP]
- transcription regulatory region DNA binding [IDA]
Mus musculus
PREY
ETL4
6620402G01Rik, 9430077C05Rik, BC026657, E330027G05Rik, Etl-4, Skt, RP23-436G18.1
enhancer trap locus 4
GO Process (1)
GO Function (0)
GO Component (1)
Gene Ontology Biological Process
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A direct physical interaction between Nanog and Sox2 regulates embryonic stem cell self-renewal.
Embryonic stem (ES) cell self-renewal efficiency is determined by the Nanog protein level. However, the protein partners of Nanog that function to direct self-renewal are unclear. Here, we identify a Nanog interactome of over 130 proteins including transcription factors, chromatin modifying complexes, phosphorylation and ubiquitination enzymes, basal transcriptional machinery members, and RNA processing factors. Sox2 was identified as a robust ... [more]
EMBO J. Jul. 26, 2013; 0(0); [Pubmed: 23892456]
Quantitative Score
- 324.0 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- used nuclear extracts, Flag-tagged interactor A
Curated By
- BioGRID