BAIT

NANOG

2410002E02Rik, ENK, ecat4
Nanog homeobox
Mus musculus

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A direct physical interaction between Nanog and Sox2 regulates embryonic stem cell self-renewal.

Gagliardi A, Mullin NP, Ying Tan Z, Colby D, Kousa AI, Halbritter F, Weiss JT, Felker A, Bezstarosti K, Favaro R, Demmers J, Nicolis SK, Tomlinson SR, Poot RA, Chambers I

Embryonic stem (ES) cell self-renewal efficiency is determined by the Nanog protein level. However, the protein partners of Nanog that function to direct self-renewal are unclear. Here, we identify a Nanog interactome of over 130 proteins including transcription factors, chromatin modifying complexes, phosphorylation and ubiquitination enzymes, basal transcriptional machinery members, and RNA processing factors. Sox2 was identified as a robust ... [more]

EMBO J. Jul. 26, 2013; 0(0); [Pubmed: 23892456]

Quantitative Score

  • 543.0 [Confidence Score]

Throughput

  • High Throughput

Additional Notes

  • used nuclear extracts, Flag-tagged interactor A

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NANOG ARID3B
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-

Curated By

  • BioGRID