An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

The F-box protein FBXO25 promotes the proteasome-dependent degradation of ELK-1.

Teixeira FR, Manfiolli AO, Soares CS, Baqui MM, Koide T, Gomes MD

FBXO25 is one of the sixty-nine known human F-box proteins that serve as specificity factors for a family of ubiquitin ligases composed of SKP1, Rbx1, Cullin1 and F-box protein (SCF1) that are involved in targeting proteins for degradation across the ubiquitin proteasome system (UPS). However, the substrates of most SCF E3 ligases remain unknown. Here, we applied an in chip ... [more]

J. Biol. Chem. Aug. 12, 2013; 0(0); [Pubmed: 23940030]

Throughput

High Throughput

Ontology Terms

cell type: hek-293t cell (BTO:0002181)

Additional Notes

The reactions were prepared as following purified SCF1FBXO25 or SCF1FBXO25 Fbox and 100 ng E1 , 500 ng E2 UbcH5c or 500 ng E2DN
To identify FBXO25 substrate candidates, we have used the human protoarray as target and the purified SCF1FBXO25 complexes as a source of E3 ligases. putative substrates of FBXO25 were identified with high confidence

Curated By

BioGRID

Interaction Summary

FBXO25

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]ubiquitin-protein transferase activity [NAS]

Gene Ontology Cellular Component

DCX

Gene Ontology Biological Process

Gene Ontology Molecular Function

microtubule binding [IDA]protein binding [IPI]protein kinase binding [IPI]