BAIT
ATP4A
ATP6A
ATPase, H+/K+ exchanging, alpha polypeptide
GO Process (3)
GO Function (0)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
PREY
VAMP2
SYB2, VAMP-2
vesicle-associated membrane protein 2 (synaptobrevin 2)
GO Process (29)
GO Function (9)
GO Component (25)
Gene Ontology Biological Process
- Golgi to plasma membrane protein transport [ISS]
- calcium ion-dependent exocytosis [ISS]
- cellular protein metabolic process [TAS]
- cellular response to insulin stimulus [ISS]
- energy reserve metabolic process [TAS]
- eosinophil degranulation [IMP]
- exocytosis [IBA, TAS]
- glutamate secretion [TAS]
- long-term synaptic potentiation [ISS]
- membrane fusion [ISS]
- membrane organization [TAS]
- mucus secretion [IMP]
- natural killer cell degranulation [IMP]
- neurotransmitter secretion [TAS]
- neutrophil degranulation [IMP]
- positive regulation of intracellular protein transport [ISS]
- post-Golgi vesicle-mediated transport [TAS]
- protein complex assembly [ISS]
- protein transport [ISS]
- regulation of exocytosis [ISS]
- regulation of histamine secretion by mast cell [IMP]
- regulation of insulin secretion [TAS]
- regulation of vesicle-mediated transport [ISS]
- response to glucose [ISS]
- small molecule metabolic process [TAS]
- synaptic transmission [TAS]
- synaptic vesicle exocytosis [ISS, TAS]
- vesicle fusion [IBA]
- vesicle-mediated transport [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- SNARE complex [IDA, TAS]
- clathrin-coated vesicle [IDA]
- clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane [TAS]
- clathrin-sculpted glutamate transport vesicle membrane [TAS]
- clathrin-sculpted monoamine transport vesicle membrane [TAS]
- cytoplasmic vesicle [ISS]
- endocytic vesicle membrane [TAS]
- extracellular vesicular exosome [IDA]
- integral component of plasma membrane [TAS]
- intracellular membrane-bounded organelle [ISS]
- membrane [IDA]
- neuron projection [ISS]
- neuron projection terminus [ISS]
- perinuclear region of cytoplasm [ISS]
- plasma membrane [ISS, TAS]
- secretory granule [ISS]
- secretory granule membrane [IDA, TAS]
- synapse [ISS]
- synaptic vesicle [IBA, ISS, TAS]
- synaptic vesicle membrane [ISS]
- synaptobrevin 2-SNAP-25-syntaxin-1a complex [ISS]
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex [ISS]
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex [ISS]
- trans-Golgi network [ISS]
- zymogen granule membrane [ISS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Characterization of immunoisolated human gastric parietal cells tubulovesicles: identification of regulators of apical recycling.
Gastric parietal cells possess an amplified apical membrane recycling system dedicated to regulated apical recycling of H-K-ATPase. While amplified in parietal cells, apical recycling is critical to polarized secretory processes in most epithelial cells. To clarify putative regulators of apical recycling, we prepared immunoisolated parietal cell H-K-ATPase-containing recycling membranes from human stomachs and analyzed protein contents by tryptic digestion and ... [more]
Am. J. Physiol. Gastrointest. Liver Physiol. May. 01, 2007; 292(5);G1249-62 [Pubmed: 17255364]
Throughput
- Low Throughput
Ontology Terms
- fundic mucosa (BTO:0004109)
Curated By
- BioGRID