An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Characterization of immunoisolated human gastric parietal cells tubulovesicles: identification of regulators of apical recycling.

Lapierre LA, Avant KM, Caldwell CM, Ham AJ, Hill S, Williams JA, Smolka AJ, Goldenring JR

Gastric parietal cells possess an amplified apical membrane recycling system dedicated to regulated apical recycling of H-K-ATPase. While amplified in parietal cells, apical recycling is critical to polarized secretory processes in most epithelial cells. To clarify putative regulators of apical recycling, we prepared immunoisolated parietal cell H-K-ATPase-containing recycling membranes from human stomachs and analyzed protein contents by tryptic digestion and ... [more]

Am. J. Physiol. Gastrointest. Liver Physiol. May. 01, 2007; 292(5);G1249-62 [Pubmed: 17255364]

Throughput

Low Throughput

Ontology Terms

tissue: fundic mucosa (BTO:0004109)

Curated By

BioGRID

Interaction Summary

ATP4A

Gene Ontology Biological Process

Gene Ontology Cellular Component

AHNAK

Gene Ontology Biological Process

Gene Ontology Molecular Function

S100 protein binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]structural molecule activity conferring elasticity [ISS]