ATP4A
Gene Ontology Biological Process
Gene Ontology Cellular Component
KCNE2
Gene Ontology Biological Process
- cardiac muscle cell action potential involved in contraction [IMP]
- cellular response to drug [IDA]
- membrane repolarization [IDA]
- membrane repolarization during action potential [IMP]
- positive regulation of proteasomal protein catabolic process [IDA]
- potassium ion export [IDA]
- potassium ion import [IMP]
- potassium ion transmembrane transport [IDA]
- regulation of delayed rectifier potassium channel activity [IDA]
- regulation of heart rate by cardiac conduction [IMP]
- regulation of inward rectifier potassium channel activity [IDA]
- regulation of membrane repolarization [IDA]
- regulation of potassium ion transmembrane transport [IDA]
- regulation of ventricular cardiac muscle cell membrane repolarization [IMP]
- ventricular cardiac muscle cell action potential [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Characterization of immunoisolated human gastric parietal cells tubulovesicles: identification of regulators of apical recycling.
Gastric parietal cells possess an amplified apical membrane recycling system dedicated to regulated apical recycling of H-K-ATPase. While amplified in parietal cells, apical recycling is critical to polarized secretory processes in most epithelial cells. To clarify putative regulators of apical recycling, we prepared immunoisolated parietal cell H-K-ATPase-containing recycling membranes from human stomachs and analyzed protein contents by tryptic digestion and ... [more]
Throughput
- Low Throughput
Ontology Terms
- tissue: fundic mucosa (BTO:0004109)
Curated By
- BioGRID