BAIT

KEG

KEEP ON GOING, T6I14.60, T6I14_60, AT5G13530
E3 ubiquitin-protein ligase KEG
Arabidopsis thaliana (Columbia)
PREY

ABF3

DC3 PROMOTER-BINDING FACTOR 5, DPBF5, F17I5.200, abscisic acid responsive elements-binding factor 3, AT4G34000
abscisic acid responsive elements-binding factor 3
Arabidopsis thaliana (Columbia)

Biochemical Activity (Ubiquitination)

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

ABA and the ubiquitin E3 ligase KEEP ON GOING affect proteolysis of the Arabidopsis thaliana transcription factors ABF1 and ABF3.

Chen YT, Liu H, Stone S, Callis J

ABF/AREB bZIP type transcription factors are positive effectors of ABA responses. Here we examine proteolytic regulation of two members, Arabidopsis thaliana ABF1 and ABF3. Both transcription factors are unstable in seedlings, and their degradation is sensitive to proteasome inhibition. ABA treatment of seedlings leads to their rapid accumulation, the result of slowed proteolysis. Deletion of the conserved C-terminal region required ... [more]

Plant J. Jun. 06, 2013; 0(0); [Pubmed: 23742014]

Throughput

  • Low Throughput

Additional Notes

  • e1-yeast
  • e2-ubc8

Curated By

  • BioGRID