A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Spd1 accumulation causes genome instability independently of ribonucleotide reduction but functions to protect the genome when deoxynucleotide pools are elevated.

Fleck O, Vejrup-Hansen R, Watson A, Carr AM, Nielsen O, Holmberg C

Cullin4, Ddb1, and Cdt2 are core subunits of the ubiquitin ligase complex CRL4(Cdt2), which controls genome stability by targeting Spd1 for degradation during DNA replication and repair in fission yeast. Spd1 has an inhibitory effect on ribonucleotide reductase (RNR), the activity of which is required for deoxynucleotide (dNTP) synthesis. Failure to degrade Spd1 in CRL4(Cdt2) defective mutants leads to DNA ... [more]

J. Cell. Sci. Aug. 28, 2013; 0(0); [Pubmed: 23986475]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112) [resistance to chemicals (APO:0000087)]

Additional Notes

MMS and CPT

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SPD1 CDC22	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hakansson P (2006)	Low	-	BioGRID	-
CDC22 SPD1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hakansson P (2006)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SPD1

Gene Ontology Biological Process

Gene Ontology Molecular Function

enzyme inhibitor activity [IDA, IGI]protein anchor [IGI]protein binding [IPI]protein complex binding [IDA]

Gene Ontology Cellular Component

CDC22