BAIT

ATARCA

RACK1A, RACK1A_AT, RECEPTOR FOR ACTIVATED C KINASE 1 A, T10F20.9, WD-40 REPEAT PROTEIN ATARCA, AT1G18080
guanine nucleotide-binding protein subunit beta-like protein A
Arabidopsis thaliana (Columbia)
PREY

AT4G00870

A_TM018A10.7, A_TM018A10_7, T18A10.17, T18A10_17
transcription factor bHLH14
GO Process (1)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Arabidopsis scaffold protein RACK1A interacts with diverse environmental stress and photosynthesis related proteins.

Kundu N, Dozier U, Deslandes L, Somssich IE, Ullah H

Scaffold proteins are known to regulate important cellular processes by interacting with multiple proteins to modulate molecular responses. RACK1 (Receptor for Activated C Kinase 1) is a WD-40 type scaffold protein, conserved in eukaryotes, from Chlamydymonas to plants and humans, expresses ubiquitously and plays regulatory roles in diverse signal transduction and stress response pathways. Here we present the use of ... [more]

Plant Signal Behav Feb. 22, 2013; 8(5); [Pubmed: 23435172]

Throughput

  • High Throughput

Curated By

  • BioGRID