Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

LESION SIMULATING DISEASE1 Interacts with Catalases to Regulate Hypersensitive Cell Death in Arabidopsis.

Li Y, Chen L, Mu J, Zuo J

LESION SIMULATING DISEASE1 (LSD1) is an important negative regulator of programmed cell death (PCD) in Arabidopsis (Arabidopsis thaliana). The loss-of-function mutations in LSD1 cause runaway cell death triggered by reactive oxygen species (ROS). LSD1 encodes a novel zinc finger protein with unknown biochemical activities. Here, we report the identification of CATALASE3 (CAT3) as an LSD1-interacting protein by affinity purification and ... [more]

Plant Physiol. Aug. 19, 2013; 0(0); [Pubmed: 23958864]

Throughput

  • Low Throughput

Additional Notes

  • CAT1 (At1g20630)
  • LSD1 (At4g20380)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AT4G20380 CAT1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
CAT1 AT4G20380
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID