An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

ELOVL1 production of C24 acyl-CoAs is linked to C24 sphingolipid synthesis.

Ohno Y, Suto S, Yamanaka M, Mizutani Y, Mitsutake S, Igarashi Y, Sassa T, Kihara A

Very long-chain fatty acids (VLCFAs) exert a variety of cellular functions and are associated with numerous diseases. However, the precise pathway behind their elongation has remained elusive. Moreover, few regulatory mechanisms for VLCFAs synthesis have been identified. Elongases catalyze the first of four steps in the VLCFA elongation cycle; mammals have seven elongases (ELOVL1-7). In the present study, we determined ... [more]

Proc. Natl. Acad. Sci. U.S.A. Oct. 26, 2010; 107(43);18439-44 [Pubmed: 20937905]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

CERS2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]sphingosine N-acyltransferase activity [IBA]

Gene Ontology Cellular Component

ELOVL6