BAIT

UBQ3

F15A17.270, F15A17_270, POLYUBIQUITIN 3 PROTEIN, polyubiquitin 3, AT5G03240
polyubiquitin 3
GO Process (3)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

ATMPK8

T10F20.15, AT1G18150
mitogen-activated protein kinase 8
GO Process (5)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Advanced Proteomic Analyses Yield a Deep Catalog of Ubiquitylation Targets in Arabidopsis.

Kim DY, Scalf M, Smith LM, Vierstra RD

The posttranslational addition of ubiquitin (Ub) profoundly controls the half-life, interactions, and/or trafficking of numerous intracellular proteins. Using stringent two-step affinity methods to purify Ub-protein conjugates followed by high-sensitivity mass spectrometry, we identified almost 950 ubiquitylation substrates in whole Arabidopsis thaliana seedlings. The list includes key factors regulating a wide range of biological processes, including metabolism, cellular transport, signal transduction, ... [more]

Plant Cell May. 13, 2013; 0(0); [Pubmed: 23667124]

Throughput

  • High Throughput

Curated By

  • BioGRID