BAIT

ADRA1D

ADRA1, ADRA1A, ADRA1R, ALPHA1, DAR, dJ779E11.2
adrenoceptor alpha 1D
Homo sapiens
PREY

SNTB1

59-DAP, A1B, BSYN2, DAPA1B, SNT2, SNT2B1, TIP-43
syntrophin, beta 1 (dystrophin-associated protein A1, 59kDa, basic component 1)
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Blood pressure is regulated by an alpha1D-adrenergic receptor/dystrophin signalosome.

Lyssand JS, DeFino MC, Tang XB, Hertz AL, Feller DB, Wacker JL, Adams ME, Hague C

Hypertension is a cardiovascular disease associated with increased plasma catecholamines, overactivation of the sympathetic nervous system, and increased vascular tone and total peripheral resistance. A key regulator of sympathetic nervous system function is the alpha(1D)-adrenergic receptor (AR), which belongs to the adrenergic family of G-protein-coupled receptors (GPCRs). Endogenous catecholamines norepinephrine and epinephrine activate alpha(1D)-ARs on vascular smooth muscle to stimulate ... [more]

J. Biol. Chem. Jul. 04, 2008; 283(27);18792-800 [Pubmed: 18468998]

Throughput

  • Low Throughput

Ontology Terms

  • cell line: hek-293 cell (BTO:0000007) [epithelial cell (CL:0000066)]

Additional Notes

  • exogenous expression of bait

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ADRA1D SNTB1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
888731

Curated By

  • BioGRID