An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

Structural and functional characterization of the N-terminus of Schizosaccharomyces pombe Cwf10.

Livesay SB, Collier SE, Bitton DA, Baehler J, Ohi MD

The spliceosome is a dynamic macromolecular machine that catalyzes the removal of introns from pre-mRNA, yielding mature message. Schizosaccharomyces pombe Cwf10 (homolog of Saccharomyces cerevisiae Snu114 and human U5-116K), an integral member of the U5 snRNP, is a GTPase that has multiple roles within the splicing cycle. Cwf10/Snu114 family members are highly homologous to eukaryotic translation elongation factor EF2, and ... [more]

Eukaryotic Cell Sep. 06, 2013; 0(0); [Pubmed: 24014766]

Throughput

High Throughput

Additional Notes

1D liquid chromatography-tandem mass spectrometry

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CDC5 SMD2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Carnahan RH (2005)	Low	-	BioGRID	-
CDC5 SMD2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	McDonald WH (1999)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CDC5

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

SMD2