An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

Structural and functional characterization of the N-terminus of Schizosaccharomyces pombe Cwf10.

Livesay SB, Collier SE, Bitton DA, Baehler J, Ohi MD

The spliceosome is a dynamic macromolecular machine that catalyzes the removal of introns from pre-mRNA, yielding mature message. Schizosaccharomyces pombe Cwf10 (homolog of Saccharomyces cerevisiae Snu114 and human U5-116K), an integral member of the U5 snRNP, is a GTPase that has multiple roles within the splicing cycle. Cwf10/Snu114 family members are highly homologous to eukaryotic translation elongation factor EF2, and ... [more]

Eukaryotic Cell Sep. 06, 2013; 0(0); [Pubmed: 24014766]

Throughput

High Throughput

Additional Notes

1D liquid chromatography-tandem mass spectrometry

Curated By

BioGRID

Interaction Summary

CDC5

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

PRP43