Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Inhibition of SAPK2a/p38 prevents hnRNP A0 phosphorylation by MAPKAP-K2 and its interaction with cytokine mRNAs.

Rousseau S, Morrice N, Peggie M, Campbell DG, Gaestel M, Cohen P

Lipopolysaccharide (LPS) stimulates production of inflammatory mediators, partly by stabilizing [interleukin-6 (IL-6), cyclooxygenase 2 (COX-2)] and/or stimulating translation [tumour necrosis factor-alpha (TNF-alpha)] of their mRNAs. Such regulation depends on AU-rich elements (AREs) within the 3'-untranslated regions and is partially suppressed by SB 203580 (which inhibits SAPK2a/p38). The LPS-induced production of TNF-alpha and IL-6 is suppressed in MAPKAP-K2-deficient mice (a kinase ... [more]

EMBO J. Dec. 02, 2002; 21(23);6505-14 [Pubmed: 12456657]

Throughput

  • Low Throughput

Ontology Terms

  • raw-264.7 cell (BTO:0003292) [liposaccharide (CHEBI:35740)]

Additional Notes

  • LPS stimulated RAW cells, inhibited by SB 203580

Curated By

  • BioGRID