BAIT

ESR1

ER, ESR, ESRA, ESTRR, Era, NR3A1, RP1-130E4.1
estrogen receptor 1
GO Process (22)
GO Function (16)
GO Component (6)
Homo sapiens
PREY

ZDHHC7

DHHC7, SERZ-B, SERZ1, ZNF370
zinc finger, DHHC-type containing 7
GO Process (2)
GO Function (1)
GO Component (5)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

DHHC-7 and -21 are palmitoylacyltransferases for sex steroid receptors.

Pedram A, Razandi M, Deschenes RJ, Levin ER

Classical estrogen, progesterone, and androgen receptors (ERs, PRs, and ARs) localize outside the nucleus at the plasma membrane of target cells. From the membrane, the receptors signal to activate kinase cascades that are essential for the modulation of transcription and nongenomic functions in many target cells. ER, PR, and AR trafficking to the membrane requires receptor palmitoylation by palmitoylacyltransferase (PAT) ... [more]

Mol. Biol. Cell Jan. 01, 2012; 23(1);188-99 [Pubmed: 22031296]

Throughput

  • Low Throughput

Additional Notes

  • The estrogen receptor alpha is likely palmyitoylated by the ZDHHC7 and ZDHHC21 PATs

Curated By

  • BioGRID