BAIT

ZFC3H1

CCDC131, PSRC2
zinc finger, C3H1-type containing
GO Process (0)
GO Function (2)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

HTT

HD, IT15
huntingtin
Alzheimer's Disease Project
Homo sapiens

Affinity Capture-Luminescence

An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.

Publication

Development and application of a DNA microarray-based yeast two-hybrid system.

Suter B, Fontaine JF, Yildirimman R, Rasko T, Schaefer MH, Rasche A, Porras P, Vazquez-Alvarez BM, Russ J, Rau K, Foulle R, Zenkner M, Saar K, Herwig R, Andrade-Navarro MA, Wanker EE

The yeast two-hybrid (Y2H) system is the most widely applied methodology for systematic protein-protein interaction (PPI) screening and the generation of comprehensive interaction networks. We developed a novel Y2H interaction screening procedure using DNA microarrays for high-throughput quantitative PPI detection. Applying a global pooling and selection scheme to a large collection of human open reading frames, proof-of-principle Y2H interaction screens ... [more]

Nucleic Acids Res. Feb. 01, 2013; 41(3);1496-507 [Pubmed: 23275563]

Throughput

  • Low Throughput

Additional Notes

  • Bait protein bound to N-terminal portion of of Huntingtin

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HTT ZFC3H1
Affinity Capture-Luminescence
Affinity Capture-Luminescence

An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.

Low-BioGRID
903981

Curated By

  • BioGRID