WDR36
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GNAQ
Gene Ontology Biological Process
- action potential [IBA]
- activation of phospholipase C activity [TAS]
- adenylate cyclase-activating G-protein coupled receptor signaling pathway [IBA]
- blood coagulation [TAS]
- glutamate receptor signaling pathway [IBA]
- negative regulation of protein kinase activity [IMP]
- phospholipase C-activating dopamine receptor signaling pathway [IBA]
- platelet activation [TAS]
- positive regulation of GTPase activity [IDA]
- protein stabilization [IMP]
- regulation of catenin import into nucleus [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
WDR36 acts as a scaffold protein tethering a G-protein-coupled receptor, Gαq and phospholipase Cβ in a signalling complex.
We identified the WD-repeat-containing protein, WDR36, as an interacting partner of the β isoform of thromboxane A(2) receptor (TPβ) by yeast two-hybrid screening. We demonstrated that WDR36 directly interacts with the C-terminus and the first intracellular loop of TPβ by in vitro GST-pulldown assays. The interaction in a cellular context was observed by co-immunoprecipitation, which was positively affected by TPβ ... [more]
Throughput
- Low Throughput
Additional Notes
- TP receptor agonist U46619 increases interaction approximately 2-fold
Curated By
- BioGRID