BAIT

GRB2

ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2
growth factor receptor-bound protein 2
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A proteomics strategy to elucidate functional protein-protein interactions applied to EGF signaling.

Blagoev B, Kratchmarova I, Ong SE, Nielsen M, Foster LJ, Mann M

Mass spectrometry-based proteomics can reveal protein-protein interactions on a large scale, but it has been difficult to separate background binding from functionally important interactions and still preserve weak binders. To investigate the epidermal growth factor receptor (EGFR) pathway, we employ stable isotopic amino acids in cell culture (SILAC) to differentially label proteins in EGF-stimulated versus unstimulated cells. Combined cell lysates ... [more]

Nat. Biotechnol. Mar. 01, 2003; 21(3);315-8 [Pubmed: 12577067]

Throughput

  • High Throughput

Ontology Terms

  • cell line: hela cell (BTO:0000567)

Additional Notes

  • GST fused to the SH2 domain of Grb2 binds activated, phosphorylated EGFR and associated binders

Curated By

  • BioGRID