BAIT

MLLT3

2210011H10Rik, 2610012I03Rik, 3830408D16Rik, Af9, D4Ertd321e, RP23-440D4.1
myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3
Mus musculus
PREY

RNF2

AI326319, AI450156, AU019207, Ring1B, dinG
ring finger protein 2
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Physical and Functional Interaction of Rnf2 with Af9 Regulates Basal and Aldosterone-stimulated Transcription of the α-ENaC Gene in a Renal Collecting Duct Cell Line.

Yu ZY, Kong Q, Kone BC

The physical and functional interaction of RING finger protein 2 (Rnf2), a central component of the Polycomb repressive complex (PRC) 1, and ALL1-fused gene from chromosome 9 protein (Af9), an aldosterone-sensitive transcription factor, in regulating basal and aldosterone-stimulated transcription of the α-ENaC (epithelial Na+ channel α-subunit) gene was explored in mIMCD3 collecting duct cells. Since Rnf2 lacks DNA-specific binding activity, ... [more]

Biosci. Rep. Sep. 27, 2013; 0(0); [Pubmed: 24070375]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MLLT3 RNF2
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID