NEK6
Gene Ontology Biological Process
- G2 DNA damage checkpoint [IMP]
- chromosome segregation [IDA]
- cytokinesis [TAS]
- mitotic cell cycle [TAS]
- mitotic nuclear envelope disassembly [TAS]
- peptidyl-serine phosphorylation [IDA]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IMP]
- protein autophosphorylation [IDA]
- protein phosphorylation [IDA]
- regulation of cellular senescence [TAS]
- regulation of mitotic cell cycle [TAS]
- regulation of mitotic metaphase/anaphase transition [IDA]
- signal transduction [IMP]
- spindle assembly [TAS]
Gene Ontology Molecular Function
CDC42
Gene Ontology Biological Process
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- GTP catabolic process [TAS]
- Golgi organization [ISS]
- T cell costimulation [TAS]
- actin cytoskeleton organization [IDA]
- axon guidance [TAS]
- blood coagulation [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- establishment of Golgi localization [ISS]
- establishment or maintenance of cell polarity [TAS]
- innate immune response [TAS]
- macrophage differentiation [TAS]
- muscle cell differentiation [TAS]
- negative regulation of epidermal growth factor receptor signaling pathway [TAS]
- negative regulation of protein complex assembly [IPI]
- organelle transport along microtubule [ISS]
- positive regulation of cytokinesis [IMP]
- positive regulation of muscle cell differentiation [TAS]
- positive regulation of pseudopodium assembly [IDA]
- positive regulation of substrate adhesion-dependent cell spreading [IDA]
- regulation of attachment of spindle microtubules to kinetochore [IMP]
- regulation of filopodium assembly [IDA]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
- substantia nigra development [IEP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi membrane [ISS]
- cytoplasm [IDA]
- cytoplasmic ribonucleoprotein granule [IDA]
- cytosol [TAS]
- extracellular vesicular exosome [IDA]
- filopodium [IDA]
- focal adhesion [IDA]
- membrane [IDA]
- midbody [IDA]
- mitotic spindle [IDA]
- neuron projection [IDA]
- neuronal cell body [IDA]
- plasma membrane [IDA, TAS]
- spindle midzone [IDA]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome.
Physical protein-protein interactions are fundamental to all biological processes and are organized in complex networks. One branch of the kinome network is the evolutionarily conserved NIMA-related serine/threonine kinases (Neks). Most of the 11 mammalian Neks studied so far are related to cell cycle regulation, and due to association with diverse human pathologies, Neks are promising chemotherapeutic targets. Human Nek6 was ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NEK6 CDC42 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID