BAIT

FBXL7

FBL6, FBL7
F-box and leucine-rich repeat protein 7
UPS Project
Homo sapiens
PREY

AHCYL1

DCAL, IRBIT, PPP1R78, PRO0233, XPVKONA
adenosylhomocysteinase-like 1
GO Process (1)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Parallel SCF Adaptor Capture Proteomics Reveals a Role for SCF(FBXL17) in NRF2 Activation via BACH1 Repressor Turnover.

Tan MK, Lim HJ, Bennett EJ, Shi Y, Harper JW

Modular cullin-RING E3 ubiquitin ligases (CRLs) use substrate binding adaptor proteins to specify target ubiquitylation. Many of the ∼200 human CRL adaptor proteins remain poorly studied due to a shortage of efficient methods to identify biologically relevant substrates. Here, we report the development of parallel adaptor capture (PAC) proteomics and its use to systematically identify candidate targets for the leucine-rich ... [more]

Mol. Cell Oct. 10, 2013; 52(1);9-24 [Pubmed: 24035498]

Throughput

  • Low Throughput

Curated By

  • BioGRID