BAIT

ETS1

ETS-1, EWSR2, p54
v-ets avian erythroblastosis virus E26 oncogene homolog 1
Cell Cycle - Senescence Project
Homo sapiens
PREY

C17ORF62

chromosome 17 open reading frame 62
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

A comprehensive resource of interacting protein regions for refining human transcription factor networks.

Miyamoto-Sato E, Fujimori S, Ishizaka M, Hirai N, Masuoka K, Saito R, Ozawa Y, Hino K, Washio T, Tomita M, Yamashita T, Oshikubo T, Akasaka H, Sugiyama J, Matsumoto Y, Yanagawa H

Large-scale data sets of protein-protein interactions (PPIs) are a valuable resource for mapping and analysis of the topological and dynamic features of interactome networks. The currently available large-scale PPI data sets only contain information on interaction partners. The data presented in this study also include the sequences involved in the interactions (i.e., the interacting regions, IRs) suggested to correspond to ... [more]

PLoS ONE Mar. 03, 2010; 5(2);e9289 [Pubmed: 20195357]

Throughput

  • Low Throughput

Additional Notes

  • Interaction from HTP screen was verified by pull-downs from in vitro translated proteins

Curated By

  • BioGRID