Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

A comprehensive resource of interacting protein regions for refining human transcription factor networks.

Miyamoto-Sato E, Fujimori S, Ishizaka M, Hirai N, Masuoka K, Saito R, Ozawa Y, Hino K, Washio T, Tomita M, Yamashita T, Oshikubo T, Akasaka H, Sugiyama J, Matsumoto Y, Yanagawa H

Large-scale data sets of protein-protein interactions (PPIs) are a valuable resource for mapping and analysis of the topological and dynamic features of interactome networks. The currently available large-scale PPI data sets only contain information on interaction partners. The data presented in this study also include the sequences involved in the interactions (i.e., the interacting regions, IRs) suggested to correspond to ... [more]

PLoS ONE Mar. 03, 2010; 5(2);e9289 [Pubmed: 20195357]

Throughput

  • Low Throughput

Additional Notes

  • Interaction from HTP screen was verified by pull-downs from in vitro translated proteins

Curated By

  • BioGRID