An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

The protein interaction landscape of the human CMGC kinase group.

Varjosalo M, Keskitalo S, Van Drogen A, Nurkkala H, Vichalkovski A, Aebersold R, Gstaiger M

Cellular information processing via reversible protein phosphorylation requires tight control of the localization, activity, and substrate specificity of protein kinases, which to a large extent is accomplished by complex formation with other proteins. Despite their critical role in cellular regulation and pathogenesis, protein interaction information is available for only a subset of the 518 human protein kinases. Here we present ... [more]

Cell Rep Apr. 25, 2013; 3(4);1306-20 [Pubmed: 23602568]

Throughput

Low Throughput

Additional Notes

BiFC assay

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SRPK2 PABPC4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Varjosalo M (2013)	High	-	BioGRID	906641

Curated By

BioGRID

Interaction Summary

SRPK2

Gene Ontology Biological Process

Gene Ontology Molecular Function

14-3-3 protein binding [ISS]ATP binding [IDA]magnesium ion binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]protein serine/threonine kinase activity [IDA]

Gene Ontology Cellular Component

PABPC4

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]poly(A) binding [IDA]poly(C) RNA binding [IDA]poly(U) RNA binding [IDA]protein binding [IPI]