An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The protein interaction landscape of the human CMGC kinase group.

Varjosalo M, Keskitalo S, Van Drogen A, Nurkkala H, Vichalkovski A, Aebersold R, Gstaiger M

Cellular information processing via reversible protein phosphorylation requires tight control of the localization, activity, and substrate specificity of protein kinases, which to a large extent is accomplished by complex formation with other proteins. Despite their critical role in cellular regulation and pathogenesis, protein interaction information is available for only a subset of the 518 human protein kinases. Here we present ... [more]

Cell Rep Apr. 25, 2013; 3(4);1306-20 [Pubmed: 23602568]

Throughput

High Throughput

Ontology Terms

hek-293 cell (BTO:0000007)

Additional Notes

Exogenous expresssion of bait

Curated By

BioGRID

Interaction Summary

CLK3

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]protein serine/threonine kinase activity [ISS]

Gene Ontology Cellular Component

PABPC1

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]poly(A) binding [IDA, TAS]poly(U) RNA binding [IDA]protein C-terminus binding [IPI]protein binding [IPI]translation activator activity [TAS]