BAIT

CDK18

PCTAIRE, PCTAIRE3, PCTK3, RP11-288G13.1
cyclin-dependent kinase 18
Alzheimer's Disease Project
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens
PREY

GNB4

CMTD1F, RP11-145M9.4
guanine nucleotide binding protein (G protein), beta polypeptide 4
UPS Project
GO Process (4)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The protein interaction landscape of the human CMGC kinase group.

Varjosalo M, Keskitalo S, Van Drogen A, Nurkkala H, Vichalkovski A, Aebersold R, Gstaiger M

Cellular information processing via reversible protein phosphorylation requires tight control of the localization, activity, and substrate specificity of protein kinases, which to a large extent is accomplished by complex formation with other proteins. Despite their critical role in cellular regulation and pathogenesis, protein interaction information is available for only a subset of the 518 human protein kinases. Here we present ... [more]

Cell Rep Apr. 25, 2013; 3(4);1306-20 [Pubmed: 23602568]

Throughput

  • High Throughput

Ontology Terms

  • cell line: hek-293 cell (BTO:0000007)

Additional Notes

  • Exogenous expresssion of bait

Curated By

  • BioGRID