An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

The protein interaction landscape of the human CMGC kinase group.

Varjosalo M, Keskitalo S, Van Drogen A, Nurkkala H, Vichalkovski A, Aebersold R, Gstaiger M

Cellular information processing via reversible protein phosphorylation requires tight control of the localization, activity, and substrate specificity of protein kinases, which to a large extent is accomplished by complex formation with other proteins. Despite their critical role in cellular regulation and pathogenesis, protein interaction information is available for only a subset of the 518 human protein kinases. Here we present ... [more]

Cell Rep Apr. 25, 2013; 3(4);1306-20 [Pubmed: 23602568]

Throughput

High Throughput

Additional Notes

Substrate (Hit) was protein microarray; all hits had Z-score greater than 0.25

Curated By

BioGRID

Interaction Summary

SRPK2

Gene Ontology Biological Process

Gene Ontology Molecular Function

14-3-3 protein binding [ISS]ATP binding [IDA]magnesium ion binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]protein serine/threonine kinase activity [IDA]

Gene Ontology Cellular Component

ARHGAP12