PTGER1
Gene Ontology Biological Process
Gene Ontology Molecular Function
DRD1
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IDA, IMP]
- G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger [ISO]
- activation of adenylate cyclase activity [IDA, ISO]
- adenylate cyclase-activating G-protein coupled receptor signaling pathway [ISO]
- adenylate cyclase-activating dopamine receptor signaling pathway [IDA, IGI, IMP, ISO]
- adult walking behavior [IGI, IMP]
- associative learning [IMP, ISO]
- astrocyte development [IMP]
- behavioral fear response [IMP]
- behavioral response to cocaine [IMP, ISO]
- calcium-mediated signaling [ISO]
- cellular response to catecholamine stimulus [ISO]
- cerebral cortex GABAergic interneuron migration [IMP]
- conditioned taste aversion [IMP]
- dentate gyrus development [IMP]
- dopamine receptor signaling pathway [IGI, IMP, ISO]
- dopamine transport [IMP]
- feeding behavior [IGI]
- glucose import [IMP]
- grooming behavior [IMP]
- habituation [IMP]
- hippocampus development [IMP]
- intracellular protein transport [ISO]
- learning [IMP]
- locomotory behavior [IGI, IMP, ISO]
- long term synaptic depression [IMP]
- long-term synaptic potentiation [IMP]
- maternal behavior [IMP]
- mating behavior [IDA]
- memory [IMP]
- muscle contraction [IMP]
- negative regulation of cell migration [ISO]
- negative regulation of circadian sleep/wake cycle, sleep [ISO]
- negative regulation of protein kinase activity [ISO]
- neuronal action potential [IMP]
- operant conditioning [IMP, ISO]
- peristalsis [IGI]
- phosphatidylinositol catabolic process [ISO]
- phosphatidylinositol metabolic process [ISO]
- phospholipase C-activating dopamine receptor signaling pathway [IDA, IGI, ISO]
- positive regulation of adenylate cyclase activity [ISO]
- positive regulation of cAMP biosynthetic process [ISO]
- positive regulation of cell migration [IMP]
- positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway [ISO]
- positive regulation of feeding behavior [ISO]
- positive regulation of long-term synaptic potentiation [ISO]
- positive regulation of membrane potential [ISO]
- positive regulation of release of sequestered calcium ion into cytosol [ISO]
- positive regulation of synaptic transmission, glutamatergic [IMP]
- prepulse inhibition [IMP]
- protein import into nucleus [IMP]
- regulation of dopamine metabolic process [IMP]
- regulation of ion transport [ISO]
- regulation of long-term neuronal synaptic plasticity [ISO]
- regulation of vasoconstriction [ISO]
- response to amphetamine [IGI, IMP]
- response to cocaine [IMP]
- response to drug [IDA, IMP]
- sensitization [IGI]
- social behavior [ISO]
- startle response [IMP]
- striatum development [IMP]
- synaptic transmission [IDA]
- synaptic transmission, dopaminergic [IMP, ISO]
- temperature homeostasis [IMP]
- transmission of nerve impulse [IMP]
- visual learning [IMP, ISO]
Gene Ontology Molecular Function- ATPase binding [ISO]
- D3 dopamine receptor binding [ISO]
- G-protein alpha-subunit binding [ISO]
- G-protein coupled receptor activity [IDA, ISO]
- angiotensin receptor binding [ISO]
- dopamine binding [ISO]
- dopamine neurotransmitter receptor activity [IDA, ISO]
- dopamine neurotransmitter receptor activity, coupled via Gs [IDA, IMP, ISO]
- drug binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein heterodimerization activity [ISO]
- protein phosphatase binding [ISO]
- receptor binding [ISO]
- ATPase binding [ISO]
- D3 dopamine receptor binding [ISO]
- G-protein alpha-subunit binding [ISO]
- G-protein coupled receptor activity [IDA, ISO]
- angiotensin receptor binding [ISO]
- dopamine binding [ISO]
- dopamine neurotransmitter receptor activity [IDA, ISO]
- dopamine neurotransmitter receptor activity, coupled via Gs [IDA, IMP, ISO]
- drug binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein heterodimerization activity [ISO]
- protein phosphatase binding [ISO]
- receptor binding [ISO]
Gene Ontology Cellular Component
- axon [ISO]
- axon terminus [ISO]
- caveola [ISO]
- cell [IMP]
- cytosol [ISO]
- dendrite [ISO]
- dendritic shaft [ISO]
- dendritic spine [ISO]
- dendritic spine head [ISO]
- dendritic spine neck [ISO]
- endoplasmic reticulum [ISO]
- integral component of membrane [ISO]
- intracellular [IMP]
- membrane [IDA]
- neuronal cell body [ISO]
- nucleus [IDA]
- plasma membrane [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Prostaglandin E receptor EP1 forms a complex with dopamine D1 receptor and directs D1-induced cAMP production to adenylyl cyclase 7 through mobilizing G(βγ) subunits in human embryonic kidney 293T cells.
The mechanism underlying the crosstalk between multiple G protein-coupled receptors remains poorly understood. We previously reported that prostaglandin E receptor EP1 facilitates dopamine D1 receptor signaling in striatal slices and promotes behavioral responses induced by D1 receptor agonists. Here, using human embryonic kidney (HEK)-293T cells expressing D1 and EP1, we have analyzed the mechanism underlying EP1-mediated facilitation of D1 receptor ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DRD1 PTGER1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID