Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Comprehensive proteomic analysis of interphase and mitotic 14-3-3-binding proteins.

Meek SE, Lane WS, Piwnica-Worms H

14-3-3 proteins regulate the cell division cycle and play a pivotal role in blocking cell cycle advancement after activation of the DNA replication and DNA damage checkpoints. Here we describe a global proteomics analysis to identify proteins that bind to 14-3-3s during interphase and mitosis. 14-3-3-binding proteins were purified from extracts of interphase and mitotic HeLa cells using specific peptide ... [more]

J. Biol. Chem. Jul. 30, 2004; 279(31);32046-54 [Pubmed: 15161933]

Throughput

  • High Throughput

Curated By

  • BioGRID