BAIT

SLC3A2

4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE
solute carrier family 3 (amino acid transporter heavy chain), member 2
Homo sapiens
PREY

SLC7A8

AA408822, LAT2
solute carrier family 7 (cationic amino acid transporter, y+ system), member 8
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine.

Rossier G, Meier C, Bauch C, Summa V, Sordat B, Verrey F, Kuehn LC

Glycoprotein-associated amino acid transporters (gpaAT) are permease-related proteins that require heterodimerization to express their function. So far, four vertebrate gpaATs have been shown to associate with 4F2hc/CD98 for functional expression, whereas one gpaAT specifically associates with rBAT. In this study, we characterized a novel gpaAT, LAT2, for which mouse and human cDNAs were identified by expressed sequence tag data base ... [more]

J. Biol. Chem. Dec. 03, 1999; 274(49);34948-54 [Pubmed: 10574970]

Throughput

  • Low Throughput

Additional Notes

  • Interaction was detected in Xenopus oocytes

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SLC7A8 SLC3A2
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
907714

Curated By

  • BioGRID