An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic identification of common SCF ubiquitin ligase FBXO6-interacting glycoproteins in three kinds of cells.

Liu B, Zheng Y, Wang TD, Xu HZ, Xia L, Zhang J, Wu YL, Chen GQ, Wang LS

FBOX6 ubiquitin ligase complex is involved in the endoplasmic reticulum-associated degradation pathway by mediating the ubiquitination of glycoproteins. FBXO6 interacts with the chitobiose in unfolded N-glycoprotein, pointing glycoproteins toward E2 for ubiquitination. Although the glycoprotein-recognizing mechanism of FBXO6 is well documented, its bona fide interacting glycoproteins are largely unknown. Here we utilized a protein purification approach combined with LC-MS to ... [more]

J. Proteome Res. Mar. 02, 2012; 11(3);1773-81 [Pubmed: 22268729]

Throughput

High Throughput

Ontology Terms

cell line: jurkat cell (BTO:0000661)
cell line: hela cell (BTO:0000567)
disease: cervical adenocarcinoma (DOID:3702)
disease: t-cell leukemia (DOID:715)

Additional Notes

exogenous expression of bait

Curated By

BioGRID

Interaction Summary

FBXO6

Gene Ontology Biological Process

Gene Ontology Molecular Function

glycoprotein binding [ISS]protein binding [IPI]

Gene Ontology Cellular Component

YWHAG

Gene Ontology Biological Process

Gene Ontology Molecular Function

insulin-like growth factor receptor binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]protein kinase C binding [IPI]protein kinase C inhibitor activity [NAS]